Analysis and stability study of temozolomide using capillary electrophoresis

Andrasi, Melinda and Bustos, Rose and Gaspar, Attila and Gomez, Frank A. and Klekner, Almos (2010) Analysis and stability study of temozolomide using capillary electrophoresis. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878 (21). pp. 1801-1808. ISSN 1570-0232

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Abstract

The applicability of micellar electrokinetic capillary chromatography (MEKC) for the analysis of temozolomide (TMZ) and its degradants, 3-methyl-(triazen-1-yl)imidazole-4-carboxamide (MTIC) and 5-amino-imidazole-4-carboxamide (AIC) has been studied. Using short-end injection, the analysis of TMZ and its degradants could be performed within 1.2 min. The obtained precision of migration times was better than 1.6 RSD%, and the limit of quantitation (LOQ) was 0.31–0.93ug/mL. The therapeutic concentration of TMZ in blood samples can be determined after direct sample injection and conventional on-capillary UV detection. The proposed MEKC method was applied to study the stability of TMZ in water and serum at different pH values. It was established that the half-life of the TMZ in vitro serum at room temperature was 33 min, close to the half-life (28 min) obtained in water at pH 7.9.

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