Utilization of KASP technology for wheat improvement

Kaur, Bhavjot and Mavi, G. S. and Gill, Manpartik S. and Saini, Dinesh Kumar (2020) Utilization of KASP technology for wheat improvement. CEREAL RESEARCH COMMUNICATIONS, 48 (4). pp. 409-421. ISSN 0133-3720 (print), 1788-9170 (online)

Text CRC_48_409-421.pdf Restricted to Repository staff only Download (1MB)

Abstract

Hundreds of experiments involving genotyping and phenotyping of the plants are planned daily by plant researchers worldwide for wheat improvement. There are a plethora of molecular markers available that are being widely used in wheat genetic studies but have their own limitations. Developments in the areas of next-generation sequencing and bioinformatics applications have aided in the development of SNP-based markers. SNP-based assays have revolutionized the method of genotyping as these consume less time and have high accuracy and efectiveness. Among the SNP genotyping platforms, kompetitive allele-specifc PCR (KASP) assay is a promising technology for high throughput SNP genotyping which is based on the fuorescence resonance energy transfer (FRET) method. KASP genotyping mainly involves two allele-specifc forward primers, one common reverse primer and FRET cassettes which results in the identifcation of respective alleles having a particular SNP or InDel. It is playing a signifcant role in performing various genetic studies such as genetic diversity analysis, genome-wide association studies and marker assisted selection in wheat. In this review, for the frst time, we attempt to give an overview of the KASP assays developed for various biotic and abiotic stresses, agronomic, physiological and quality traits for wheat improvement and the assays developed for genetic diversity analysis and genotyping by sequencing.

Actions (login required)

Edit Item