Nitrogen stress-induced TaDof1 expression in diverse wheat genotypes and its relation with nitrogen use efficiency

Bharati, Alka and Tehlan, Gayatri and Nagar, Chetan Kumar and Sinha, Subodh Kumar and Venkatesh, Karnam and Mandal, Pranab Kumar (2022) Nitrogen stress-induced TaDof1 expression in diverse wheat genotypes and its relation with nitrogen use efficiency. CEREAL RESEARCH COMMUNICATIONS, 50 (4). pp. 637-645. ISSN 0133-3720 (print), 1788-9170 (online)

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Abstract

Nitrogen (N) uptake and nitrogen utilization are two major components of nitrogen use efficiency (NUE), where N-utilization is a function of N-assimilation and N-remobilization. Transcription factor Dof1 regulates Carbon (C) metabolism in the TCA cycle and also controls the NUE in the plant by ensuring optimum C-skeleton supply for N-assimilation. In this study, four diverse wheat genotypes were selected based on their varied response to N stress in a field experiment conducted during 2012–2013. These genotypes are subjected to N-stress in Pots in the next two consecutive years (2014, 2015), where N stress level on year 2 (2015) was higher than year 1 (2014). Expression of Tadof1 was studied in leaf tissue from two growth stages, 30 (stage 1) and 60 (stage 2), days after sowing. TaDof1 expression was found upregulated during year 1 and gradually down-regulated at stage 2 of year 2 when the N-stress was maximum. HS-277, an Efficient NUtE genotype, shows relatively highest TaDof1 expression under limited N-stress; however, the downregulation was also maximum under severe stress. In optimum N supply, GS2 (Glutamine synthetase) and NADH-GOGAT (Glutamate synthase) activity increased in wheat leaves when the plants grew up from stage 1 to stage 2. However, the activity reduced significantly under severe N-stress in the low NUtE genotypes, Sujata and VL-401. Our result shows that role of TaDof1 varies among the wheat genotypes, and this variation is probably one of the factors affecting N-assimilation and ultimately yield as well as NUE.

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