Characterization of Xanthomonas arboricola pv. juglandis isolates in Hungary

Végh, Anita and Fodor, Attila and Békési, Orsolya and Szani, Zsolt and Bujdosó, Géza (2023) Characterization of Xanthomonas arboricola pv. juglandis isolates in Hungary. In: 12th INTERNATIONAL CONGRESS OF PLANT PATHOLOGY, 20-25, August 2023, France, Lyon.

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Abstract

Xanthomonas arboricola pv. juglandis is the causal agent of walnut blight, the most important bacterial disease of Juglans species, which affects a high percentage of pistillate flowers and fruits, but does not kill bearing trees. Symptoms of the disease consist of dark brown to black spots on new leaves, stems and nuts. Many nuts fall prematurely; others reach full size, but their kernel become blackened, dried and wrinkled. In Hungary, walnut blight disease occur and cause important damage in gardens and orchards. Since 2020, we have been constantly monitoring the symptoms. The collected leaf and fruits samples were decontaminated, homogenized and streaked on King B agar. After 24 hours of incubation at 26 °C the Gram property of isolates was determined by KOH test. We examined all isolates to induce a hypersensitive reaction on tobacco leaves. Biochemical API test was also used for identification. Finally, the pathogenicity of the isolates was checked. In the case of walnut blight, immature walnut fruits and leaves were artificial infected with a bacterial suspension. For molecular identification of the isolates, the 16S rDNA region were amplified using a universal primer pair (63F, 1389R). Xanthomonas arboricola pv. juglandis isolates that cause walnut blight disease have been identified by classical and molecular methods in Hungary.

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