Optical Waveguide Light-Mode Spectroscopy for Ion Channel Profiling

Székács, Inna (2015) Optical Waveguide Light-Mode Spectroscopy for Ion Channel Profiling. METHODS IN PHARMACOLOGY AND TOXICOLOGY, 53. pp. 155-165. ISSN 1557-2153

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Abstract

Ion channel-based biosensors using label-free optical waveguide light-mode spectroscopy (OWLS) technique provide a sensitive measurement method of trans-channel ion transport, and allow further development in utilization of ion channels as models for pharmacological purposes (drug design targeting ion channels or diagnostic applications in clinical trials). This chapter describes a sensor setup for supported cell- derived membrane fragments deposited onto a hydrophilic polytetrafluoroethylene membrane with further separation from the OWLS sensor surface by a thin polyethylene terephthalate membrane. This approach provides spatial separation between the lipid layer and the sensor surface, and also allows space for possible extramembranous domains of the inbuilt membrane channel proteins. Influx of Cl− ions through GABAA channels in the presence or absence of GABA and channel blocking agent bicuculline is measured by changes of the optical characteristics in the evanescent field at near proximity of the OWLS sensor surface.

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