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SUMOylation of phytochrome-B negatively regulates light-induced signaling in Arabidopsis thaliana.

Sadanandom, Ari and Ádám, Éva and Orosa, Beatriz and Viczián, András and Klose, Cornelia and Kozma-Bognár, László and Nagy, Ferenc István (2015) SUMOylation of phytochrome-B negatively regulates light-induced signaling in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 112 (35). pp. 11108-11113. ISSN 0027-8424

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Abstract

The red/far red light absorbing photoreceptor phytochrome-B (phyB) cycles between the biologically inactive (Pr, lambdamax, 660 nm) and active (Pfr; lambdamax, 730 nm) forms and functions as a light quality and quantity controlled switch to regulate photomorphogenesis in Arabidopsis. At the molecular level, phyB interacts in a conformation-dependent fashion with a battery of downstream regulatory proteins, including PHYTOCHROME INTERACTING FACTOR transcription factors, and by modulating their activity/abundance, it alters expression patterns of genes underlying photomorphogenesis. Here we report that the small ubiquitin-like modifier (SUMO) is conjugated (SUMOylation) to the C terminus of phyB; the accumulation of SUMOylated phyB is enhanced by red light and displays a diurnal pattern in plants grown under light/dark cycles. Our data demonstrate that (i) transgenic plants expressing the mutant phyB(Lys996Arg)-YFP photoreceptor are hypersensitive to red light, (ii) light-induced SUMOylation of the mutant phyB is drastically decreased compared with phyB-YFP, and (iii) SUMOylation of phyB inhibits binding of PHYTOCHROME INTERACTING FACTOR 5 to phyB Pfr. In addition, we show that OVERLY TOLERANT TO SALT 1 (OTS1) de-SUMOylates phyB in vitro, it interacts with phyB in vivo, and the ots1/ots2 mutant is hyposensitive to red light. Taken together, we conclude that SUMOylation of phyB negatively regulates light signaling and it is mediated, at least partly, by the action of OTS SUMO proteases.

Item Type: Article
Subjects: Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 12 Oct 2015 10:03
Last Modified: 13 Oct 2015 07:16
URI: http://real.mtak.hu/id/eprint/29714

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