REAL

Retromer ensures the degradation of autophagic cargo via maintaining lysosome function in Drosophila

Maruzs, Tamás and Lőrincz, Péter and Szatmári, Zsuzsanna and Széplaki, Szilvia and Sándor, Zoltán and Juhász, Gábor (2015) Retromer ensures the degradation of autophagic cargo via maintaining lysosome function in Drosophila. TRAFFIC, 16 (10). pp. 1088-1107. ISSN 1398-9219

[img] Text
MaruzsTraffic.pdf
Restricted to Registered users only

Download (20MB) | Request a copy

Abstract

The retromer is an evolutionarily conserved coat complex that consists of Vps26, Vps29, Vps35 and a heterodimer of sorting nexin (Snx) protein in yeast. Retromer mediates the recycling of transmembrane proteins from endosomes to the trans-Golgi network, including receptors that are essential for the delivery of hydrolytic enzymes to lysosomes. Besides its function in lysosomal enzyme receptor recycling, involvement of retromer has also been proposed in a variety of vesicular trafficking events, including early steps of autophagy and endocytosis. Here we show that the late stages of autophagy and endocytosis are impaired in Vps26 and Vps35 deficient Drosophila larval fat body cells, but formation of autophagosomes and endosomes is not compromised. Accumulation of aberrant autolysosomes and amphisomes in the absence of retromer function appears to be the consequence of decreased degradative capacity, as they contain undigested cytoplasmic material. Accordingly, we show that retromer is required for proper cathepsin L trafficking mainly independent of LERP, the Drosophila homolog of the cation-independent mannose 6-phosphate receptor. Finally, we find that Snx3 and Snx6 are also required for proper autolysosomal degradation in Drosophila larval fat body cells.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 25 Apr 2016 13:42
Last Modified: 25 Apr 2016 13:42
URI: http://real.mtak.hu/id/eprint/34889

Actions (login required)

Edit Item Edit Item