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Transcriptome profile of early responsive genes in susceptible barley during Rhynchosporium secalis infection

Aldaoude, A. and Shoaib, A. and Al-Shehadah, E. and Rajeh, M. and Jawhar, M. and Arabi, M.I.E. (2016) Transcriptome profile of early responsive genes in susceptible barley during Rhynchosporium secalis infection. Cereal Research Communications, 44 (1). pp. 153-161. ISSN 0133-3720

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Abstract

Scald caused by Rhynchosporium secalis, is an economically important disease found worldwide. In order to profile genes and pathways responding to R. seclais infection, leaf transcriptomes before and after fungus inoculation in susceptible barley were compared using cDNA-AFLP technique. Transcriptional changes of 144 expressed sequence tags (ESTs) were observed, of which 18 have no previously described function. Functional annotation of the transcripts revealed a wide range of pathways including cell wall fortification, cytoskeleton construction and metabolic processes at different time points. Furthermore, the results of RT-PCR analysis on candidate genes, ABC transporters and lycine-specific demethylase were consistent with the cDNA-AFLP data in their expression patterns. Taken together, our data suggest that susceptible barley reprograms metabolic and biological processes to initiate a suitable response R. secalis infection.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
Depositing User: Ágnes Sallai
Date Deposited: 07 Jun 2016 08:34
Last Modified: 31 Mar 2017 23:15
URI: http://real.mtak.hu/id/eprint/35758

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