Identification of new SSR markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat under water stressed condition

Barakat, Mohamed and Saleh, Mohamed and Al-Doss, Abdullah and Moustafa, Khaled and Elshafei, Adel and Al-Qurainy, Fahed (2015) Identification of new SSR markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat under water stressed condition. Acta Biologica Hungarica, 66 (1). pp. 93-102. ISSN 0236-5383

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Abstract

Segregating F₄ families from the cross between drought sensitive (Yecora Rojo) and drought tolerant (Pavon 76) genotypes were made to identify SSR markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat (Triticum aestivum L.) under water-stressed condition and to map quantitative trait locus (QTL) for the three physiological traits. The parents and 150 F₄ families were evaluated phenotypically for drought tolerance using two irrigation treatments (2500 and 7500 m³/ha). Using 400 SSR primers tested for polymorphism in testing parental and F₄ families genotypes, the results revealed that QTL for leaf chlorophyll content, flag leaf senescence and cell membrane stability traits were associated with 12, 5 and 12 SSR markers, respectively and explained phenotypic variation ranged from 6 to 42%. The SSR markers for physiological traits had genetic distances ranged from 12.5 to 25.5 cM. These SSR markers can be further used in breeding programs for drought tolerance in wheat.

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