REAL

Immune response to gut Escherichia coli and susceptibility to adjuvant arthritis in the rats

Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana (2015) Immune response to gut Escherichia coli and susceptibility to adjuvant arthritis in the rats. Acta Microbiologica et Immunologica Hungarica, 62 (1). pp. 1-19. ISSN 1217-8950

[img]
Preview
Text
amicr.62.2015.1.1.pdf

Download (416kB) | Preview

Abstract

We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of E. coli in molecular weight range between 20–30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to E. coli, especially of the IgG2b antibody class. Intramuscular administration of E. coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3<sup>+</sup>CD26<sup>+</sup> cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats’ genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 30 Jun 2016 11:29
Last Modified: 01 Jul 2016 07:17
URI: http://real.mtak.hu/id/eprint/36890

Actions (login required)

Edit Item Edit Item