The role of pancreatic ductal secretion in protection against acute pancreatitis in mice*.

Pallagi, Petra and Balla, Zsolt and Singh, Anurag K. and Dósa, Sándor and Iványi, Béla and Kukor, Zoltán and Tóth, Adél and Riederer, Brigitte and Liu, Yongjian and Engelhardt, Regina and Jármay, Katalin and Szabó, Andrea and Janovszky, Agnes and Perides, George and Venglovecz, Viktória and Maléth, József and Wittmann, Tibor and Takács, Tamás and Gray, Mike A. and Gácser, Attila and Hegyi, Péter and Seidler, Ursula and Rakonczay, Zoltán (2014) The role of pancreatic ductal secretion in protection against acute pancreatitis in mice*. Critical care medicine, 42 (3). e177-88. ISSN 1530-0293

Text 00003246-201403000-00048.pdf Restricted to Registered users only Download (5MB) | Request a copy

Abstract

OBJECTIVES A common potentially fatal disease of the pancreas is acute pancreatitis, for which there is no treatment. Most studies of this disorder focus on the damage to acinar cells since they are assumed to be the primary target of multiple stressors affecting the pancreas. However, increasing evidence suggests that the ducts may also have a crucial role in induction of the disease. To test this hypothesis, we sought to determine the specific role of the duct in the induction of acute pancreatitis using well-established disease models and mice with deletion of the Na/H exchanger regulatory factor-1 that have selectively impaired ductal function. DESIGN Randomized animal study. SETTING Animal research laboratory. SUBJECTS Wild-type and Na/H exchanger regulatory factor-1 knockout mice. INTERVENTIONS Acute necrotizing pancreatitis was induced by i.p. administration of cerulein or by intraductal administration of sodium taurocholate. The pancreatic expression of Na/H exchanger regulatory factor-1 and cystic fibrosis transmembrane conductance regulator (a key player in the control of ductal secretion) was analyzed by immunohistochemistry. In vivo pancreatic ductal secretion was studied in anesthetized mice. Functions of pancreatic acinar and ductal cells as well as inflammatory cells were analyzed in vitro. MEASUREMENTS AND MAIN RESULTS Deletion of Na/H exchanger regulatory factor-1 resulted in gross mislocalization of cystic fibrosis transmembrane conductance regulator, causing marked reduction in pancreatic ductal fluid and bicarbonate secretion. Importantly, deletion of Na/H exchanger regulatory factor-1 had no deleterious effect on functions of acinar and inflammatory cells. Deletion of Na/H exchanger regulatory factor-1, which specifically impaired ductal function, increased the severity of acute pancreatitis in the two mouse models tested. CONCLUSIONS Our findings provide the first direct evidence for the crucial role of ductal secretion in protecting the pancreas from acute pancreatitis and strongly suggest that improved ductal function should be an important modality in prevention and treatment of the disease.

Actions (login required)

Edit Item