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Computational analysis of translational readthrough proteins in Drosophila and yeast reveals parallels to alternative splicing

Pancsa, Rita and Macossay-Castillo, Mauricio and Kosol, Simone and Tompa, Péter (2016) Computational analysis of translational readthrough proteins in Drosophila and yeast reveals parallels to alternative splicing. SCIENTIFIC REPORTS, 6. p. 32142. ISSN 2045-2322

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Abstract

In translational readthrough (TR) the ribosome continues extending the nascent protein beyond the first in-frame termination codon. Due to the lack of dedicated analyses of eukaryotic TR cases, the associated functional-evolutionary advantages are still unclear. Here, based on a variety of computational methods, we describe the structural and functional properties of previously proposed D. melanogaster and S. cerevisiae TR proteins and extensions. We found that in D. melanogaster TR affects long proteins in mainly regulatory roles. Their TR-extensions are structurally disordered and rich in binding motifs, which, together with their cell-type-and developmental stage-dependent inclusion, suggest that similarly to alternatively spliced exons they rewire cellular interaction networks in a temporally and spatially controlled manner. In contrast, yeast TR proteins are rather short and fulfil mainly housekeeping functions, like translation. Yeast extensions usually lack disorder and linear motifs, which precludes elucidating their functional relevance with sufficient confidence. Therefore we propose that by being much more restricted and by lacking clear functional hallmarks in yeast as opposed to fruit fly, TR shows remarkable parallels with alternative splicing. Additionally, the lack of conservation of TR extensions among orthologous TR proteins suggests that TR-mediated functions may be generally specific to lower taxonomic levels. © The Author(s) 2016.

Item Type: Article
Uncontrolled Keywords: YEAST; structure activity relation; exon; DROSOPHILA; developmental stage; chemical binding; cell interaction; alternative RNA splicing
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 05 Oct 2016 12:32
Last Modified: 05 Oct 2016 12:32
URI: http://real.mtak.hu/id/eprint/41629

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