A survey for bvdv antibodies in cattle farms in Slovakia and genetic typing of bvdv isolates from imported animals

Vilček, Š. and Mojžišová, Jana and Bajová, Viera and Paulík, Š. and Strojný, L. and Ďurkovič, B. and Hipíková, Vlasta (2003) A survey for bvdv antibodies in cattle farms in Slovakia and genetic typing of bvdv isolates from imported animals. Acta Veterinaria Hungarica, 51 (2). pp. 229-236. ISSN 0236-6290

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Abstract

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5´-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1h group.

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