Endometrial mRNA expression of prostaglandin synthase enzymes PTGS 2, PTGFS and mPTGES 1 in repeat-breeding cows with cytologically determined endometritis

Janowski, Tomasz and Barański, Wojciech and Łukasik, Karolina and Skarżyński, Dariusz and Zduńczyk, Sławomir and Malinowska, Katarzyna (2017) Endometrial mRNA expression of prostaglandin synthase enzymes PTGS 2, PTGFS and mPTGES 1 in repeat-breeding cows with cytologically determined endometritis. Acta Veterinaria Hungarica, 65 (1). pp. 96-104. ISSN 0236-6290

Preview

Text 004.2017.010.pdf Download (219kB) | Preview

Abstract

Little is known about the inflammatory response of the endometrium in repeat-breeding cows with subclinical endometritis (SE). The objective of this study was to evaluate the mRNA expression of prostaglandin-endoperoxide synthase 2 (PTGS 2), prostaglandin F2_α synthase (PTGFS) and prostaglandin E2 microsomal synthase 1 (mPTGES 1) in the endometrium of repeat-breeding cows with and without SE. SE was diagnosed cytologically using the cytobrush method, with the threshold being set at 5% polymorphonuclear neutrophils. Biopsy samples were obtained from the endometrium of repeat-breeding cows with SE (n = 10) and without SE (n = 10). The mRNA expression of the synthases was evaluated using qRT-PCR. Significantly higher (P < 0.05) expression of the PTGS 2 gene was detected in the repeat breeders with SE, whereas there was no significant difference in the expression of PTGFS and mPTGES 1 mRNAs between repeatbreeding cows with SE and those without it (P > 0.05). Our study confirms that increased endometrial expression of the PTGS 2 gene is involved in the inflammatory response in repeat breeders.

Actions (login required)

Edit Item