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Novel and sensitive qPCR assays for the detection and identification of aspergillosis causing species

Paholcsek, Melinda and Leiter, Éva and Markovics, Arnold and Biró, Sándor (2014) Novel and sensitive qPCR assays for the detection and identification of aspergillosis causing species. Acta Microbiologica et Immunologica Hungarica, 61 (3). pp. 273-284. ISSN 1217-8950

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Abstract

Despite concerted efforts, diagnosis of aspergillosis is still a great challenge to clinical microbiology laboratories. Along with the requirement for high sensitivity and specificity, species-specific identification is important. We developed rapid, sensitive and species-specific qPCR assays using the TaqMan technology for the detection and identification of Aspergillus fumigatus and Aspergillus terreus. The assays were designed to target orthologs of the Streptomyces factor C gene that are only found in a few species of filamentous fungi. Fungi acquired this gene through horizontal gene transfer and divergence of the gene allows identification of species. The assays have potential as a molecular diagnosis tool for the early detection of fungal infection caused by Aspergillus fumigatus and Aspergillus terreus, which merits future diagnostic studies. The assays were sensitive enough to detect a few genomic equivalents in blood samples.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 28 Mar 2017 12:10
Last Modified: 28 Mar 2017 12:10
URI: http://real.mtak.hu/id/eprint/50586

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