ABC transporter ATPase of Chlamydophila pneumoniae as a potential vaccine candidate

Szabó, Ágnes and Sipák, Zoltán and Miczák, András and Faludi, Ildikó (2013) ABC transporter ATPase of Chlamydophila pneumoniae as a potential vaccine candidate. Acta Microbiologica et Immunologica Hungarica, 60 (1). pp. 11-20. ISSN 1217-8950

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Abstract

Better vaccines and new therapeutic drugs could be a successful breakthrough against intracellular bacteria. M. tuberculosis ABC transporter ATPase (Rv0986) plays a role in mycobacterial virulence by inhibiting phagosome-lysosome fusion. Thus, it could be a potential vaccine candidate. C. pneumoniae another important intracellular bacterium possesses a protein named CpB0255, which is homologous with the mycobacterial Rv0986. The aim of this study was the cloning, over-expression and purification of CpB0255 ABC transporter ATPase protein to study its biological properties. The immunogenicity and protective effect of recombinant chlamydial ATPase protein combined with Alum adjuvant were investigated in mice. The immunization resulted in the reduction of the number of viable C. pneumoniae in the lungs after challenge. Our results confirm that chlamydial ATPase induces protective immunity in mice.

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