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Effectiveness of a high purity chlorine dioxide solution in eliminating intracanal Enterococcus faecalis biofilm

Herczegh, Anna and Ghidan, Ágoston and Friedreich, Dóra and Gyurkovics, Milán and Bendő, Zsolt and Lohinai, Zsolt (2013) Effectiveness of a high purity chlorine dioxide solution in eliminating intracanal Enterococcus faecalis biofilm. Acta Microbiologica et Immunologica Hungarica, 60 (1). pp. 63-75. ISSN 1217-8950

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Abstract

We investigated the effectiveness of chlorine dioxide (ClO<sub>2</sub>) solution in comparison to sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHX) in the elimination of intracanal Enterococcus faecalis biofilm. Extracted human teeth were inoculated with E. faecalis. After preparation the canals were irrigated with ClO<sub>2</sub>, NaOCl, CHX or physiologic saline for control. Two and five days later bacterial samples were collected and streaked onto Columbia agar. CFU/mL were counted. The canal walls were investigated by scanning electron microscopy (SEM). The gas phase was investigated in an upside down Petri dish where E. faecalis was inoculated onto blood agar. The irrigants were placed on absorbent paper into the cover. Bacteria were detectable in the control group, but not in any of the irrigants groups. There was a massive reinfection 2 or 5 days after irrigation in the control group. The lowest reinfection was found after the ClO<sub>2</sub> treatment. These findings were confirmed by SEM images. We observed an antibacterial effect of ClO<sub>2</sub> and NaOCl gas phases on E. faecalis growth, but not of CHX. ClO<sub>2</sub> eliminates intracanal biofilm and keeps canal nearly free from bacteria. We suggest the use of high purity ClO<sub>2</sub> as a root canal irrigant in clinical practice.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 20 Apr 2017 07:40
Last Modified: 20 Apr 2017 07:40
URI: http://real.mtak.hu/id/eprint/51541

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