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The lack of L-PG production and the repercussions of it in regards to M. Tuberculosis interactions with mononuclear phagocytes

Fol, Marek and Głobińska, Anna and Stączek, Paweł and Kowalewicz-Kulbat, Magdalena and Druszczyńska, Magdalena and Madiraju, Murty and Rudnicka, Wiesława (2013) The lack of L-PG production and the repercussions of it in regards to M. Tuberculosis interactions with mononuclear phagocytes. Acta Microbiologica et Immunologica Hungarica, 60 (2). pp. 127-144. ISSN 1217-8950

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Abstract

The lysine connection with phosphatidylglycerol (PG) alters the M. tuberculosis(Mtb) surface charge, and consequently it may decrease the bacterial vulnerability to antimicrobial action of the immune cells. The aim of the study was to assess the significance of PG lysinylation in the Mtb interactions with mononuclear phagocytes. Both the Mtb strain with deletion of lysX gene (Mtb-lysX) which is responsible for PG lysinylation as well as the complemented strain (Mtb-compl) was used to infect human blood monocytes or THP-1 cells. The monocytes were obtained by MACS technique, or THP-1 cells. The Mtb-lysX strain has exhibited the enhanced sensitivity to HNP 1-3. However, it was not susceptible to bactericidal action of cathepsin G. The LysX deletion did not influence the Mtb ability of monocyte induction to IL-10 secretion. The intra- and extracellular expression of MHC-II was similarly reduced after the Mtb-lysX or Mtb-Rv infections. Noticeably significant is that the Mtb strain with deleted lysX has not affected the intensity of the gene expression of cathepsin G compared to the uninfected monocytes. That is the clear contrast to what the Mtb-Rv strain has proved. The obtained results suggest that the Mtb ability to lysinylate PG is a participatory element in mycobacterial strategy of survival inside phagocytic cells. However, the extended studies are needed to determine its influence on the other immune cells and define its role in the developing of Mtb infection.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 20 Apr 2017 07:49
Last Modified: 20 Apr 2017 07:49
URI: http://real.mtak.hu/id/eprint/51549

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