Molecular identification of a new wheat-Thinopyrum intermedium ssp. trichophorum addition line for resistance to stripe rust

Song, X. and Li, G. and Zhan, H. and Liu, C. and Yang, Z. (2013) Molecular identification of a new wheat-Thinopyrum intermedium ssp. trichophorum addition line for resistance to stripe rust. Cereal Research Communications, 41 (2). pp. 211-220. ISSN 0133-3720

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, was one of the most disaster foliar diseases for wheat-growing areas of the world. Thinopyrum intermedium has provided novel resistance genes to multi-fungal disease, and new wheat-Th. intermedium derivatives for stripe rust resistance still need to develop for wheat breeding. Wheat line X484-3 was selected from a cross between wheat line MY11 and wheat-Th. intermedium ssp. trichophorum partial amphiploid TE-1508, and was characterized by genomic in situ hybridization (GISH) and functional molecular markers. Chromosome counting revealed that the X484-3 was 2n = 44 and GISH analysis using Pseudoroegneria spicata genomic DNAas a probe demonstrated that X484-3 contained a pair of St-chromosomes from Th. intermedium donor parents. The functional molecular markers confirmed that introduced St-chromosomes belonging to linkage group 7, indicating that line X484-3 was a 7St addition line. The resistance observation displayed that the introduced Th. intermedium ssp. trichophorum derived chromosomes 7St were responsible for the stripe rust resistances at adult plant. The identified wheat-Th. intermedium chromosome 7St addition line X484-3 can be used as a donor in wheat breeding for stripe rust resistance.

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