Molecular analysis of S-haplotypes in peach, a self-compatible Prunus species

Hegedűs, Attila and Szabó, Zoltán and Nyeki, J. and Halász, Júlia and Pedryc, Andrzej (2006) Molecular analysis of S-haplotypes in peach, a self-compatible Prunus species. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 131 (6). pp. 738-743. ISSN 0003-1062

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Abstract

The most commercially grown peach [Prunus persica (L.) Batsch.] cultivars do not require cross-pollination for reasonable fruit set; however, self-incompatibility is a well-known feature within the Prunoideae subfamily. Isoelectric focusing and native polyacrylamide gel electrophoresis of S-ribonucleases; PCR analyses of S-RNase and S-haplotype-specific F-box genes as well as DNA sequencing were carried out to survey the self- (in)compatibility allele pool and to uncover the nature of self-compatibility in peach. From 25 cultivars and hybrids with considerable diversity in phenotype and origin, only two S-haplotypes were detected. Allele identity could be checked by exact length determination of the PCR-amplified fragments and/or partial sequencing of the peach S-1-, S-2-, and Prunus davidiana (Carr.) Franch. S-1 RNases. S-RNases of peach were detected to possess ribonuclease activity, and a single nucleotide polymorphism in the S,-RNase was shown, which represents a synonymous substitution and does not change the amino acid present at the position in the protein. A 700-bp fragment of the peach SFB gene was PCR-amplified, which is similar to the fragment size of functional Prunus L. SFBs. All data obtained in this study may support the contribution of genes outside the S-locus to the self-compatible phenotype of peaches.

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