Effect of 2,4-D concentration on the androgenic response in anther culture of barley

Kruczkowska, Hanna and Pawłowska, Helena and Skucińska, Barbara (2005) Effect of 2,4-D concentration on the androgenic response in anther culture of barley. Cereal Research Communications, 33 (4). pp. 727-732. ISSN 0133-3720

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Abstract

The aim of the study was to determine the effect of various 2,4-D concentrations on the androgenic response and plant development in anther culture of barley. Anthers were either plated directly on the FHG medium supplemented with 20 mg dm ⁻³ of 2,4-D, or pretreated with 0.7 mol dm ⁻³ mannitol and plated on an induction medium containing various concentrations of 2,4-D: 0.2, 2.0, or 20 mg dm ⁻³ . The concentration of 20 mg dm ⁻³ was used for 3 days only; subsequently, anthers were transferred onto the medium containing 0.2 mg dm ⁻³ of 2,4-D. It was shown that osmotic stress cannot be replaced with the high concentration of 2,4-D. The most numerous embryos, embryogenic calli, and plants (including green plants) were obtained with 0.7mol dm ⁻³ mannitol pretreatment and 0.2 mg dm ⁻³ of 2,4-D in the induction medium. The auxin concentration also affected the dynamics of embryogenic structure formation and plant development from embryogenic structures. The concentrations higher than 0.2 mg dm ⁻³ slowed down embryogenic structure formation and plant development, and they increased the proportion of calli as compared to embryos in plant regeneration.

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