Tagging pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala

Dufresne, Marie and Lee, Theo and M’Barek, Sarrah and Xu, X. and Zhang, X. and Liu, Taiguo and Zhang, Wenwei and Kema, Gert and Daboussi, Marie-Josée and Waalwijk, Cees (2008) Tagging pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala. Cereal Research Communications, 36 (Supple). pp. 415-419. ISSN 0133-3720

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Abstract

Transposon mutagenesis was applied to generate mutants in Fusarium graminearum . The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum , making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome.

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