Evaluation of genetic variation in high molecular weight glutenin subunits of seed storage protein using landraces of common wheat from Pakistan

Niwa, K. and Suzuki, H. and Tominaga, T. and Nasim, S. and Anwar, R. and Ogawa, M. and Furuta, Y. (2008) Evaluation of genetic variation in high molecular weight glutenin subunits of seed storage protein using landraces of common wheat from Pakistan. Cereal Research Communications, 36 (2). pp. 327-332. ISSN 0133-3720

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Abstract

SDS-PAGE was used to determine the variation in high molecular weight glutenin subunits (HMW-GS) alleles present in 170 landraces of common wheat from Sindh, Balochistan and NWFP in Pakistan. Three alleles for Glu-A1 , six alleles for Glu-B1 and four alleles for Glu-D1 were detected. These included three new alleles, namely Glu-B1e, Glu-B1h and Glu-D1n , which had not previously been found in landraces of Pakistani wheat. Furthermore, Glu-D1m and Glu-D1n , which are considered to be endemic alleles in Pakistan and Afghanistan, were both detected from Balochistan. Twenty genotypes were identified based on combinations of alleles at the three Glu-1 loci. The present study showed that Pakistani landraces of common wheat have maintained a broad diversity of HMW-GS alleles, and that they may serve as genetic resources to improve wheat varieties adapted to Pakistani climatic and edaphic conditions.

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