Validation and utilisation of Rht dwarfing gene specific

Pestsova, E. and Korzun, V. and Börner, A. (2008) Validation and utilisation of Rht dwarfing gene specific. Cereal Research Communications, 36 (2). pp. 235-246. ISSN 0133-3720

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Abstract

PCR assays specific for the GA insensitive dwarfing genes (alleles) Rht-B1b and Rht-D1b were employed to study a series of additional alleles of Rht-B1 and Rht-D1 . The amplification profiles of Rht-B1b and Rht-B1d were not distinguishable from one another, whereas lines carrying Rht-B1c, Rht-B1e and Rht-B1f amplified a product analogous to that of the wild type. At the 4D locus, no discrimination was possible between Rht-D1b, Rht-D1c and Rht-D1d . As a result, the utilisation of these PCR assays is limited. Examples of the analysis of germplasm and spontaneous occurring off-types are presented.

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