Washing solutions for the determination of the western corn rootworm eggs in soil

Števo, J. and Cagáň, Ľ. (2012) Washing solutions for the determination of the western corn rootworm eggs in soil. Cereal Research Communications, 40 (1). pp. 147-156. ISSN 0133-3720

Text crc.40.2012.1.16.pdf Restricted to Repository staff only until 31 March 2032. Download (72kB)

Abstract

Thirty-three washing solutions with various molar concentrations were visually tested as potentially washing solutions for separation of the Western corn rootworm (WCR) eggs from the soil. The results showed that 1.5 or 2.0 molar solution of MgSO₄.7H₂O, 1.0 and 1.5 molar solution of ZnSO₄.7H₂O, 3.0, 4.0 and 5.0 molar solution of NaNO₃, MgCl₂.6H₂Owith molarity of 2.0, NaCl with molarity of 4.0 or 5.0, Ca(NO₃)₂.4H₂O with molarity of 1.5 and 2.0, solution of sucrose with molarity of 1.5 or 2.0, KCl with molarity of 4.0, and NaH₂PO₄.2H₂O with molarity of 2.0, 3.0 or 4.0 were considered as suitable for the separation of WCR eggs from soil and they would be useful tool in integrated pest management for monitoring the density of WCR eggs in maize fields. After separation of the eggs from soil, the eggs were tested for their viability. Percentages of hatched eggs after separation of eggs from soil by washing solutions were not significantly different to control variant. Marginal density of washing solution was determined by beaker and tube test. Marginal density of washing solution, in which the eggs started to move up towards the surface of solution was calculated at the level of 1.086929 g/cm³. As a safe solution density for the separation of the WCR eggs from soil (the density, in which all eggs completely flew to the surface), was considered the level of 1.125 g/cm³.

Actions (login required)

Edit Item