Genomewide Screen for Modulators of Evolvability under Toxic Antibiotic Exposure.

Méhi, Orsolya Katinka and Bogos, Balázs and Csörgő, Bálint and Pál, Csaba (2013) Genomewide Screen for Modulators of Evolvability under Toxic Antibiotic Exposure. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 57 (7). pp. 3453-3456. ISSN 0066-4804

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Abstract

Antibiotic resistance is generally selected within a window of concentrations high enough to inhibit wild-type growth but low enough for new resistant mutants to emerge. We studied de novo evolution of resistance to ciprofloxacin in an Escherichia coli knockout library. Five null mutations had little or no effect on intrinsic antibiotic susceptibility but increased the upper antibiotic dosage to which initially sensitive populations could adapt. These mutations affect mismatch repair, translation fidelity, and iron homeostasis.

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