Serological, Pathological and Molecular Characterisation of Hungarian Pepper Mild Mottle Tobamovirus (PMMoV) Isolates

Kálmán, D. and Palkovics, L. and Gáborjányi, R. (2001) Serological, Pathological and Molecular Characterisation of Hungarian Pepper Mild Mottle Tobamovirus (PMMoV) Isolates. Acta Phytopathologica et Entomologica Hungarica, 36 (1-2). pp. 31-42. ISSN 0238-1249

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Abstract

Last year pepper growers observed symptoms referring to virus-infection in pepper plantations in plastic tunnels. Infected plants showed mosaic symptoms or mottling of the leaves, while on the fruits necrotic spots developed. These symptoms referred to a tobamovirus infection. Collected samples were examined by serological and pathological methods, followed by the biological characterisation of the isolates. For serological studies the DAS-ELISA method was used, in which the pathogen was identified as pepper mild mottle tobamovirus. During the pathological examination different host-plants have been used including some pepper varieties containing different L genes (L + –L 4). It was found, that the Hungarian isolates belonged to the P 1,2 pathotype and were closely related to the Spanish isolate (PMMV-S). PCR- studies proved the presence of the PMMoV P 1,2 pathotype in Hungary as well.

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