Molecular genetic traditional methods for detection of Mycobacterium tuberculosis complex (Discrepanncy analysis)

Deák, Judith and Nagy, Erzsébet and Veréb, Ilona and Weszelowszky, Erzsébet and Somfay, A. and Kraszkó, P. (2001) Molecular genetic traditional methods for detection of Mycobacterium tuberculosis complex (Discrepanncy analysis). Acta Microbiologica et Immunologica Hungarica, 48 (3-4). pp. 449-455. ISSN 1217-8950

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Abstract

In the past six and half years, 862 different clinical samples [sputum, bronchoalveolar lavage, thorax puncture, cerebrospinal fluid and skin samples] were tested by Gen-probe amplified Mycobacterium tuberculosis direct test (MTD) or ligase chain reaction (LCR) or polymerase chain reaction (PCR). 239 parallel clinical samples were cultivated, and some samples were stained with Ziehl-Neelsen staining. 1-4 samples were tested per patient. 29 (12.13%) samples were positive and 177 (74.05%) samples were negative with both cultivation and molecular genetic methods. 2 (0.83%)samples were positive only on cultivation, and 31 (12.97%) samples were positive only with the molecular diagnostic methods. The differences are undoubtedly explained by the sensitivity of the molecular diagnostic methods.

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