Valproate and delta-sleep peptide display high efficacy against metaphit-induced audiogenic seizure in rats

Stanojlović, O and Hrnčic, D and Živanović, D and Rašić, A and Šušić, V (2006) Valproate and delta-sleep peptide display high efficacy against metaphit-induced audiogenic seizure in rats. Acta Physiologica Hungarica, 93 (4). pp. 303-314. ISSN 0231-424X

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Abstract

The effects of valproate (VPA) and delta sleep -inducing peptide (DSIP) on metaphit -induced generalized, audiogenic seiz ure in adult rat males were compared. The animals were i.p. injected with : 1. Saline; 2. metaphit (mp, 10mg kg–1); 3. metaphit (10mg kg–1) and 8 h later with DSIP (0.1, 0.2, 0.4 or 1.0mg kg–1 ), 4. metaphit (10mg kg–1) and 8 h later with VPA (50, 75 or 100mg kg–1); 5. DSIP alone (1.0mg kg–1) and 6. VPA, alone (100mg kg–1). The rats were exposed to sound stimulation at hourly intervals and the behavior and EEG were analyzed. The EEG signals in metaphit rats appeared as a sleep -like pattern and spike -wa ve complexes with increased power spectra. Valproate and DSIP reduced the incidence of seizure and prolonged duration of latency in a dose -dependent manner. ED50 of valproate in the 1st hour after administration was 63.19mg kg–1 and that of DSIP 3.19mg kg–1 four hours after injection. This suggests that VPA, reached a peak of action immediately after the application, while DSIP had a prolonged action, mildly reducing, but not abolishing metaphit seizure. None of the applied VPA and DSIP doses eliminated the metaphit -provoked EEG signs of epileptiform activity.

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