HISTOPHYSIOLOGICAL CHANGES OF THE TESTICULAR TISSUE DUE TO BUSULPHAN ADMINISTRATION IN THE WILD INDIAN HOUSE RAT (RATTUS RATTUS)

Aich, S. and Manna, C. K. (2001) HISTOPHYSIOLOGICAL CHANGES OF THE TESTICULAR TISSUE DUE TO BUSULPHAN ADMINISTRATION IN THE WILD INDIAN HOUSE RAT (RATTUS RATTUS). Acta Biologica Hungarica, 52 (1). pp. 105-116. ISSN 0236-5383

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Abstract

The results of the present study indicate the antispermatogenic activity of Busulphan or Myleran (1,4- dimethane-sulphonoxy butane) on the testicular tissue of adult male Indian house rat, Rattus rattus. Single oral dose of Busulphan (10 mg/Kg body weight) was administered and its activity was noticed at 10, 40, 70 and 100 days of posttreated animals. Histological observation and quantitative histological study indicates no major alteration in the rela- tive percentages of primary spermatocytes, spermatid and Sertoli cells at 10 days of posttreatment. But there was a gradual decrease in the seminiferous tubular diameter at 40 and 70 days of post treated groups. However, the Leydig and Sertoli cells morphology and number remained normal in all the treat- ment groups. At 40 days, the normal cellular associations in all the tubules were disrupted. The tubules constituted only spermatogonia, Sertoli cells and some zygotene spermatocytes. At 70 days, repopulation of Type A, Type B spermatogonia, resting and zygotene spermatocytes occurred at this stage. The tubules were still devoid of pachytene spermatocytes, spermatid and spermatozoa. At 100 days, active sper- matogenesis was observed in majority of the tubules. The various types of germ cell population were regaining towards normalcy. Histochemical studies clearly revealed that due to busulphan administration there was no major alter- ation in the intensities of some key enzymes (i.e. D5 3b-HSDH and 17b-HSDH) involved in the biosyn- thesis of steroid hormones. Only the acid phosphatase activity was slightly depressed within the 40th and 70th days of posttreatment. Sudanophilic lipid materials increased in the interstitium of all the busulphan post treated groups. The changes which were noticed due to busulphan treatment regained normalcy at 100 days of post treated animals. The mode of action of Busulphan on the testicular tissue of adult Indian house rat (Rattus rattus) has been pointed out and discussed.

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