Effect of Yellow mosaic virus on pathogenesis related enzymes and chlorophyll content in mothbean (Vigna aconitifolia)

Arora, R. and Joshi, U. N. and Gupta, P. P. and Singh, J. V. (2009) Effect of Yellow mosaic virus on pathogenesis related enzymes and chlorophyll content in mothbean (Vigna aconitifolia). Acta Phytopathologica et Entomologica Hungarica, 44 (1). pp. 49-60. ISSN 0238-1249

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Abstract

Mothbean plants (resistant and susceptible genotypes) were inoculated with Yellow mosaic virus (YMV) at 30 days after sowing (DAS), under controlled conditions in a naturally lit net house. Leaves samples were collected at 31, 33, 35, 37, 48 and 66 DAS and analysed for pathogenesis related enzymes and chlorophyll content. Yellow mosaic virus affects the activities of enzymes related to pathogenesis in mothbean even at 24 hours after inoculation. Activities of enzymes like peroxidase, polyphenol oxidase, phenylalanine ammonia lyase and tyrosine ammonia lyase were increased, whereas catalase activity was found to decrease in the leaves of inoculated plants of all the four genotypes tested, RMO 225 and HM 61 (resistant), GMO 9703 and GMO 9704 (susceptible), as compared to the uninoculated plants. Peroxidase activity increased markedly in the susceptible genotypes as compared to the resistant genotypes of inoculated plants. The activities of polyphenol oxidase showed an increasing trend in the inoculated leaves of resistant genotypes, as compared to the resistant genotypes of uninoculated ones. The results indicate that the differences between tolerance and susceptibility of hosts are quantitative as well as qualitative so defence against a pathogen entails major changes in the biochemical components of the plant. A probable role of these enzymes for defence against YMV in mothbean is discussed.

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