REAL

Low variability of Internal Transcribed Spacer rDNA and trnL (UAA) intron sequences of several taxa in the Festuca ovina aggregate (Poaceae)

Galli, Z. and Penksza, K. and Kiss, E. and Sági, L. and Heszky, L. E. (2006) Low variability of Internal Transcribed Spacer rDNA and trnL (UAA) intron sequences of several taxa in the Festuca ovina aggregate (Poaceae). Acta Biologica Hungarica, 57 (1). pp. 57-69. ISSN 0236-5383

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Abstract

Identification and classification of numerous Festucaspecies is still a difficult problem due to the close morphological resemblance. The most difficult fine fescues to identify belong to the Festucaovinaaggregate, which is the largest group in the genus Festuca. Many taxons are considered to be separate species based on quantitative taxonomic characters, differences in ploidy level or the structure of sclerenchyma cells. In order to evaluate the taxonomic value of DNA-based markers, sequence analysis of the internal transcribed spacer (ITS1-5.8S-ITS2) region and the chloroplast trnL (UAA) intron was performed in the ten most problematic fine fescues belonging to the Festuca ovinaaggregate. Intraspecific ITS variants were found in a single case while in other cases only intragenomic ITS polymorphisms were detected with 1-2 ambiguous positions. Among the sequences of the trnL (UAA) intron even intragenomic polymorphisms were not detected in any of the Festucaspecies studied. Thus, the results do not support the species status of these ten taxa.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Endre Sarvay
Date Deposited: 20 Nov 2017 14:20
Last Modified: 20 Nov 2017 14:20
URI: http://real.mtak.hu/id/eprint/70068

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