The influence of estradiol and progesterone and melatonin supplementation on TNF-α levels in ovariectomized and pinealectomized rats

Oztekin, Esma and Mogulkoc, R. and Baltaci, A. K. and Tiftik, A. M. (2006) The influence of estradiol and progesterone and melatonin supplementation on TNF-α levels in ovariectomized and pinealectomized rats. Acta Biologica Hungarica, 57 (3). pp. 275-281. ISSN 0236-5383

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Abstract

The aim of this study was to investigate the effect of estradiol and progesterone and melatonin supplementation on TNF-a levels in ovariectomized and pinealectomized rats. The study was carried out on 42 adult, Spraque-Dawley strain female rats aged 6 months and weighing 200-250 grams. The rats were divided into 6 groups, each group contained 7 rats. Group 1: Sham-ovariectomized (Sham-Ovx), Group 2: Ovariectomized (Ovx), Group 3: Ovx and estradiol (E) and progesterone (P) supplemented (Ovx+E-P) group, Group 4: Ovx+E-P+Melatonin (M) supplemented group, Group 5: Ovx - Pinealectomized (Pnx) group, Group 6: Ovx - Pnx+E-P supplemented group. Serum TNF-a levels were determined after 4 weeks application period. Group 6 (Ovx-Pnx+E-P) has the highest serum TNF-a compared with other groups while group 2 (ovariectomized), has the lowest levels (P<0.001). Group 5 was higher than groups 1, 2, 3 and 4 (P<0.001). The results of the study show that ovariectomy reduces the serum level of TNF-a, but estradiol and progesterone application prevents this reduction in ovariectomized rats. However, pinealectomy intensifies the increases in TNF- a levels in ovariectomized and estradiol and progesterone supplemented rats, whereas melatonin reduces TNF- a levels in ovariectomized rats.

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