In vitro effects of alloxan/copper combinations on lipid peroxidation, protein oxidation and antioxidant enzymes

Alexandrova, Albena and Petrov, L. and Kessiova, Mila and Kirkova, Margarita (2007) In vitro effects of alloxan/copper combinations on lipid peroxidation, protein oxidation and antioxidant enzymes. Acta Biologica Hungarica, 58 (4). pp. 359-367. ISSN 0236-5383

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Abstract

The in vitro effects of alloxan and the product of its reduction dialuric acid (alone or in combination with copper ions) on lipid peroxidation, carbonyl content, GSH level and antioxidant enzyme activities in rat liver and kidney have been studied. The effects of Cu 2+ /alloxan and Cu 2+ /dialuric acid were compared with those of Fe 3+ /alloxan and Fe 3+ /dialuric acid. Unlike alloxan, dialuric acid increased liver and kidney lipid peroxidation; similar effects were registered in the presence of Fe 3+ . In the presence of Cu 2+ /dialuric acid, the lipid peroxidation was strongly inhibited and vice versa – the liver protein oxidation was increased. Alloxan and dialuric acid, as well as their combinations with Fe 3+ had no effect on the total GSH level. Both substances did not affect the Cu 2+ -induced changes in GSH level, glucose-6-phosphate dehydrogenase and gluthatione reductase activities. In contrast, Cu 2+ had no effect on dialuric-acid induced changes in gluthatione peroxidase and superoxide dismutase activities. The present in vitro results, concerning the metal dependence of the effects of alloxan and dialuric acid, are a premise for in vivo study of alloxan effects in metal-loaded animals.

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