Changes in the Archaea microbial community when the biogas fermenters are fed with protein-rich substrates

Ács, Norbert and Kovács, Etelka and Wirth, Roland and Bagi, Zoltán and Strang, Orsolya and Herbel, Zsófia and Rákhely, Gábor and Kovács, Kornél Lajos (2013) Changes in the Archaea microbial community when the biogas fermenters are fed with protein-rich substrates. BIORESOURCE TECHNOLOGY, 131. pp. 121-127. ISSN 0960-8524

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Abstract

Terminal restriction fragment length polymorphism (T-RFLP) was applied to study the changes in the composition of the methanogens of biogas-producing microbial communities on adaptation to proteinrich monosubstrates such as casein and blood. Specially developed laboratory scale (5-L) continuously stirred tank reactors have been developed and used in these experiments. Sequencing of the appropriate T-RF fragments selected from a methanogen-specific (mcrA gene-based) library revealed that the methanogens responded to the unconventional substrates by changing the community structure. T-RFLP of the 16S rDNA gene confirmed the findings.

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