Expression of the UVR8 photoreceptor in different tissues reveals tissue-autonomous features of UV-B signalling

Bernula, Péter and Crocco, C. D. and Arongaus, A. B. and Ulm, R. and Nagy, Ferenc István and Viczián, András (2017) Expression of the UVR8 photoreceptor in different tissues reveals tissue-autonomous features of UV-B signalling. PLANT CELL AND ENVIRONMENT, 40 (7). pp. 1104-1114. ISSN 0140-7791

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Abstract

The Arabidopsis UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) orchestrates the expression of hundreds of genes, many of which can be associated with UV-B tolerance. UV-B does not efficiently penetrate into tissues, yet UV-B regulates complex growth and developmental responses. To unravel to what extent and how UVR8 located in different tissues contributes to UV-B-induced responses, we expressed UVR8 fused to the YELLOW FLUORESCENT PROTEIN (YFP) under the control of tissue-specific promoters in a uvr8 null mutant background. We show that (1) UVR8 localized in the epidermis plays a major role in regulating cotyledon expansion, and (2) expression of UVR8 in the mesophyll is important to protect adult plants from the damaging effects of UV-B. We found that UV-B induces transcription of selected genes, including the key transcriptional regulator ELONGATED HYPOCOTYL 5 (HY5), only in tissues that express UVR8. Thus, we suggest that tissue-autonomous and simultaneous UVR8 signalling in different tissues mediates, at least partly, developmental and defence responses to UV-B.

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