Gibberellic acid effects on protein pattern, hydrolytic enzyme activities and ionic uptake during germination of Vicia faba in sea water

Shukry, W. M. and El-Bassiouny, H. M. S. (2002) Gibberellic acid effects on protein pattern, hydrolytic enzyme activities and ionic uptake during germination of Vicia faba in sea water. Acta Botanica Hungarica, 44 (1-2). pp. 145-162. ISSN 0236-6495

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Abstract

The germination and water uptake of Vicia faba seeds were suppressed in response to the treatments with the different concentrations of sea water (5%, 25% and 50%). The following parameters were increased: the osmotic potential, Na+, Cl, proline and protease activity. While K+, K+/Na+ ratio, Ca2+, amylase activity and total soluble sugars were decreased. Gibberellic acid treatments to the seeds counteracted the harmful effect which were induced by sea water treatments. In turn the germination percentage, water uptake, K+, K+/Na+ ratio, Ca2+, total soluble sugars, a-amylase and protease activities were increased, while Na+, Cl, and proline were decreased. The changes in protein banding pattern in Vicia faba germinated seeds in sea water were investigated. Salinization induced de novo synthesis of some salt responsive proteins. The salt responsive proteins might be osmotin (M wt 23.39 and 26.86 KDa), dehydrin (36.79 and 40.63 KDa) and ubiquitin (8.81 KDa) which were apparent in Vicia faba seeds. The seeds priming soaking in GA and germinated in sea water induced de novo synthesis of some responsive proteins. This indicated that gibberellic acid had a synergistic effect on the induction of the salt gene which is responsible for the synthesis of the mentioned proteins.

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