Two transgenic mouse models for beta subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations

Kacsó, Gergely and Ravasz, Dóra and Dóczi, Judit and Németh, Beáta and Madgar, O. and Iordanov, Iordan and Gál, Anikó and Molnár, Mária Judit and Nagy, Zsolt and Patócs, Attila Balázs and Vizi-Ádám, Vera and Chinopoulos, Christos (2016) Two transgenic mouse models for beta subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations. BIOCHEMICAL JOURNAL, 473 (20). pp. 3463-3485. ISSN 0264-6021

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Abstract

Succinate-CoA ligase is a heterodimer enzyme composed of Suclg1 -alpha- and a substrate-specific Sucla2 or Suclg2 -beta- subunit yielding ATP or GTP, respectively. In humans, the deficiency of this enzyme leads to encephalomyopathy with, or without methylmalonyl aciduria, in addition to resulting in mitochondrial DNA depletion. We generated mice lacking either one Sucla2 or Suclg2 allele. Sucla2 heterozygote mice exhibited tissue- and age-dependent decreases in Sucla2 expression associated with decreases in ATP-forming activity, but rebound increases in cardiac Suclg2 expression and GTP-forming activity. Bioenergetic parameters including substrate-level phosphorylation were not different between wild type and Sucla2 heterozygote mice unless a submaximal pharmacological inhibition of succinate-CoA ligase was concomitantly present. mtDNA contents were moderately decreased, but blood carnitine esters were significantly elevated. Suclg2 heterozygote mice exhibited decreases in Suclg2 expression but no rebound increases in Sucla2 expression or changes in bioenergetic parameters. Surprisingly, deletion of one Suclg2 allele in Sucla2 heterozygote mice still led to a rebound but protracted increase in Suclg2 expression, yielding double heterozygote mice with no alterations in GTP-forming activity or substrate-level phosphorylation, but more pronounced changes in mtDNA content and blood carnitine esters, and an increase in succinate dehydrogenase activity. We conclude that a partial reduction in Sucla2 elicits rebound increases in Suclg2 expression which is sufficiently dominant to overcome even a concomitant deletion of one Suclg2 allele, pleiotropically affecting metabolic pathways associated with succinate-CoA ligase. These results as well as the availability of the transgenic mouse colonies will be of value in understanding succinate-CoA ligase deficiency.

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