The proteomic profile of a mouse model of proliferative vitreoretinopathy

Márkus, Bernadett and Pató, Zsuzsanna and Sarang, Zsolt and Albert, Réka and Tőzsér, József and Petrovski, Goran and Csősz, Éva (2017) The proteomic profile of a mouse model of proliferative vitreoretinopathy. FEBS OPEN BIO, 7 (8). pp. 1166-1177. ISSN 2211-5463

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Abstract

Proliferative vitreoretinopathy (PVR) develops as a complication of retinal detachment surgery and represents a devastating condition leading to serious vision loss. A good animal model that permits extensive functional studies and drug testing is crucial in finding better therapeutic modalities for PVR. A previously established mouse model, using dispase injection, was analyzed from the proteomic point of view, examining global protein profile changes by 2D electrophoresis, image analysis and HPLC-tandem mass spectrometry-based protein identification. The easy applicability of the mouse model was used to study the role of transglutaminase 2 (TG2) in PVR formation by proteomic examination of dispase-induced TG2 knockout vitreous samples. Our data demonstrate that, despite the altered appearance of crystallin proteins, the lack of TG2 did not prevent the development of PVR.

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