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Syndecan-4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition

Keller-Pintér, Anikó and Szabó, Kitti and Kocsis, Tamás and Deák, Ferenc and Ocsovszki, Imre and Zvara, Ágnes and Puskás, László and Szilák, László and Dux, László (2018) Syndecan-4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition. FEBS LETTERS, 592 (18). pp. 3139-3151. ISSN 0014-5793

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Abstract

Myostatin, a TGF-beta superfamily member, is a negative regulator of muscle growth. Here we describe how myostatin activity is regulated by syndecan-4, a ubiquitous transmembrane heparan sulfate proteoglycan. During muscle regeneration the levels of both syndecan-4 and promyostatin decline gradually after a sharp increase, concurrently with the release of mature myostatin. Promyostatin and syndecan-4 co-immunoprecipitate, and the interaction is heparinase-sensitive. ShRNA-mediated silencing of syndecan-4 reduces C2C12 myoblast proliferation via blocking the progression from G1- to S-phase of the cell cycle, which is accompanied by elevated levels of myostatin and p21(Waf1/Cip1), and decreases in cyclin E and cyclin D1 expression. Our results suggest that syndecan-4 functions as a reservoir for promyostatin regulating the local bioavailability of mature myostatin. This article is protected by copyright. All rights reserved.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 31 Jan 2019 11:54
Last Modified: 31 Jan 2019 11:54
URI: http://real.mtak.hu/id/eprint/90831

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