Determination of Aflatoxins and Ochratoxin A in Wheat from Different Regions of Turkey by HPLC with Fluorescence Detection

Turksoy, S. and Kabak, B. (2020) Determination of Aflatoxins and Ochratoxin A in Wheat from Different Regions of Turkey by HPLC with Fluorescence Detection. Acta Alimentaria, 49 (1). pp. 118-124. ISSN 0139-3006 (print); 1588-2535 (online)

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Abstract

This study examines the occurrence of aflatoxins (AFS) and ochratoxin A (OTA) in bread and durum wheat samples. A total of 141 samples were collected from eleven different regions of Turkey. An analytical method based on liquid extraction, immunoaffinity column (IAC) clean-up followed by high performance liquid chromatography (HPLC) was used for the determination of AFs and OTA levels. As a result, AFs and OTA were detected in 2% and 9.2% of wheat samples at concentrations varying from 0.21 to 0.44 µg kg−1 and from 0.1 to 3.2 µg kg−1, respectively. Aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) were found positive in samples ranging between 0.21–0.35 µg kg−1 and 0.094 µg kg−1, respectively. However, none of the samples contained aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2). The study also recommended that contamination levels in wheat and wheat-based products should be routinely monitored in greater sample numbers to insure food safety.

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