Detection of 3′-end RNA uridylation with a protein nanopore

Clamer, Massimiliano and Höfler, Lajos and Mikhailoha, Ellina and Viero, Gabriella and Bayley, Hagan (2014) Detection of 3′-end RNA uridylation with a protein nanopore. ACS Nano, 8 (2). pp. 1364-1374. ISSN 1936-0851

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Abstract

Post-transcriptional modifications of the 3′-ends of RNA molecules have a profound impact on their stability and processing in the cell. Uridylation, the addition of uridines to 3′-ends, has recently been found to be an important regulatory signal to stabilize the tagged molecules or to direct them toward degradation. Simple and cost-effective methods for the detection of this post-transcriptional modification are not yet available. Here, we demonstrate the selective and transient binding of 3′-uridylated ssRNAs inside the β barrel of the staphylococcal α-hemolysin (αHL) nanopore and investigate the molecular basis of uridine recognition by the pore. We show the discrimination of 3′-oligouridine tails on the basis of their lengths and propose the αHL nanopore as a useful sensor for this biologically relevant RNA modification. © 2013 American Chemical Society.

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