Effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins

Bahar, Mehmet Refik and Tekin, Suat and Beytur, Asiye and Onalan, Ebru Etem and Ozyalin, Fatma and Colak, Cemil and Sandal, Süleyman (2023) Effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins. BIOLOGIA FUTURA, 74 (1-2). pp. 159-170. ISSN 2676-8615 (print); 2676-8607 (online)

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Abstract

This study was conducted to determine the possible effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins (UCPs) in rats. Forty male Wistar Albino rats were divided into 4 groups with 10 animals in each group: control, sham, 10 and 100 μM MOTS-c. Hypothalamus, blood, muscle, adipose tissues samples were collected for thyrotropin-releasing hormone (TRH), UCP1 and UCP3 levels were determined by the RT-PCR and western blot analysis. Serum thyroid hormone levels were determined by the ELISA assays. MOTS-c infusion was found to increase food con- sumption but it did not cause any changes in the body weight. MOTS-c decreased serum TSH, T3, and T4 hormone levels. On the other hand, it was also found that MOTS-c administration increased UCP1 and UCP3 levels in peripheral tissues. The findings obtained in the study show that central MOTS-c infusion is a directly effective agent in energy metabolism.

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