Bugyi, Fanni and Turiák, Lilla and Drahos, László and Tóth, Gábor (2023) Optimization of reversed-phase solid-phase extraction for shotgun proteomics analysis. JOURNAL OF MASS SPECTROMETRY. ISSN 1076-5174
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Journal of Mass Spectrometry - 2023 - Bugyi F.pdf Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Reversed-phase solid-phase extraction (SPE) is the method of choice for the purification of proteomics samples. Even though the efficacy of SPE methods is sample type-dependent, the manufacturers' protocols are used in most studies. Using an optimized SPE method can lead to a substantial gain in identification and recovery. In this tutorial, we give a brief introduction to the most important parameters influencing SPE performance, and we present a short workflow (16 measurements) for optimizing the SPE procedure. This is complemented by method performance assessment instructions and a short troubleshooting guide to help users further understand and investigate their SPE methods.
| Item Type: | Article |
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| Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
| Depositing User: | Dr. Lilla Turiák |
| Date Deposited: | 17 Sep 2023 09:30 |
| Last Modified: | 17 Sep 2023 09:30 |
| URI: | http://real.mtak.hu/id/eprint/173793 |
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