Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells

Nagy, Viktória and Mounir, Raji and Szebeni, Gábor and Szakonyi, Zsolt and Gémes, Nikolett and Minorics, Renáta and Zupkó, István (2023) Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (13). ISSN 1661-6596


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The present study aimed to characterize the antiproliferative and antimetastatic properties of two recently synthesized monoterpene-aminopyrimidine hybrids (1 and 2) on A2780 ovary cancer cells. Both agents exerted a more pronounced cell growth inhibitory action than the reference agent cisplatin, as determined by the MTT assay. Tumor selectivity was assessed using non-cancerous fibroblast cells. Hybrids 1 and 2 induced changes in cell morphology and membrane integrity in A2780 cells, as evidenced by Hoechst 33258–propidium iodide fluorescent staining. Cell cycle analysis by flow cytometry revealed substantial changes in the distribution of A2780 ovarian cancer cells, with an increased rate in the subG1 and G2/M phases, at the expense of the G1 cell population. Moreover, the tested molecules accelerated tubulin polymerization in a cell-free in vitro system. The antimetastatic properties of both tested compounds were investigated by wound healing and Boyden chamber assays after 24 and 48 h of incubation. Treatment with 1 and 2 resulted in time- and concentration-dependent inhibition of migration and invasion of A2780 cancer cells. These results support that the tested agents may be worth of further investigation as promising anticancer drug candidates.

Item Type: Article
Additional Information: Institute of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, H-6720, Hungary Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, H-6720, Hungary Laboratory of Functional Genomics, Eötvös Loránd Research Network Biological Research Centre, Institute of Genetics, Szeged, H-6726, Hungary Interdisciplinary Centre of Natural Products, University of Szeged, Szeged, H-6720, Hungary Export Date: 24 July 2023 Correspondence Address: Zupkó, I.; Institute of Pharmacodynamics and Biopharmacy, Hungary; email: Funding details: Magyar Tudományos Akadémia, MTA, BO/00582/22/8, TKP2021-EGA-32 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, 142877 FK22, 2020-1.1.6-JÖVŐ-2021-00003, K 143690 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA Funding text 1: This research was funded by the Hungarian Research Foundation (NKFI), grant numbers K 143690, 142877 FK22, and 2020-1.1.6-JÖVŐ-2021-00003, as well as by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences BO/00582/22/8 (G.J.S.). TKP2021-EGA-32 was implemented with support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme.
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
R Medicine / orvostudomány > RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
Depositing User: MTMT SWORD
Date Deposited: 26 Sep 2023 10:52
Last Modified: 26 Sep 2023 10:52

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