Aronia melanocarpa fruit juice prevents hepatic impairment in rats subjected to metabolic syndrome

Reyzov, M. and Eftimov, M. and Gancheva, S. and Todorova, M. and Zhelyazkova-Savova, M. and Tzaneva, M. and Valcheva-Kuzmanova, S. (2024) Aronia melanocarpa fruit juice prevents hepatic impairment in rats subjected to metabolic syndrome. ACTA ALIMENTARIA: AN INTERNATIONAL JOURNAL OF FOOD SCIENCE, 53 (2). pp. 270-280. ISSN 0139-3006

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Abstract

This study evaluated the effects of Aronia melanocarpa fruit juice (AMFJ) on the liver in a model of high-fat, high-fructose (HFHF) diet-induced metabolic syndrome (MS). Fifty rats were allocated to five groups – Control, MS, MS+AMFJ 2.5 , MS+AMFJ 5 , and MS+AMFJ 10 . The control group was fed a standard diet, while the other groups were provided a HFHF diet. During MS induction, daily oral treatment was performed. Control and MS groups received 10.0 mL kg −1 distilled water, while the other three groups received AMFJ at doses of 2.5, 5.0, and 10.0 mL kg −1 , respectively. After 10 weeks, liver samples were collected and inspected histologically as well as immunohistochemically to determine the expression of Bax, Bcl-2, and MAC387. Bax/Bcl-2 ratio was calculated. In MS rats, steatotic, inflammatory and degenerative alterations of the liver were detected, Bax and MAC387 were markedly elevated, while Bcl-2 was non-significantly reduced. The Bax/Bcl-2 ratio significantly increased. The histopathological alterations were prevented by the AMFJ treatment. Compared to MS group, Bax and MAC387 values were significantly lower and Bcl-2 value was higher resulting in significantly lower ( P < 0.001) Bax/Bcl-2 ratio in all AMFJ-treated groups. AMFJ, administered during MS induction in rats, prevented the occurrence of inflammatory, steatotic, degenerative, and pro-apoptotic changes in the liver.

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