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LC-MS/MS Fingerprinting Analysis of Cyanotis arachnoidea Extracts: Process-Related Artifacts in Anabolic Food Supplements

Laczkó, Dávid and Chu, En-Liang and Chang, Ching-Chia and Chang, Fang-Rong and Girst, Gábor and Gáti, Tamás and Tóth, Gábor and Könczöl, Árpád and Hunyadi, Attila (2025) LC-MS/MS Fingerprinting Analysis of Cyanotis arachnoidea Extracts: Process-Related Artifacts in Anabolic Food Supplements. ACS OMEGA, 10 (18). pp. 18605-18614. ISSN 2470-1343

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Abstract

We report the isolation and complete NMR characterization of four new ecdysteroids and LC-MS/MS fingerprinting of ecdysteroids in two native and two industrially processed Cyanotis arachnoidea extracts along with an autoxidized product mixture of 20-hydroxyecdysone (20E). The autoxidation of 20-hydroxyecdysone leads to the formation of various unknown or uncharacterized ecdysteroid compounds, which can significantly impact the quality and efficacy of commercial ecdysteroid-containing supplements, potentially affecting their regulatory status and consumer safety. A total of 146 ecdysteroids were detected. Among these, 20 were identified using authentic and fully characterized reference standards, including the newly reported compounds. The autoxidative origin of many process-related artifacts was confirmed in the two commercial ecdysteroid extracts. Considering the pharmacological versatility of ecdysteroids and the major pharmacological differences between these compounds and their autoxidized derivatives, our results are of importance regarding the efficacy and safety of commercially available ecdysteroid-containing food supplements.

Item Type: Article
Additional Information: This work was financially supported by the NKFIH, Hungary (K-134704), the Ministry of Innovation and Technology of Hungary (TKP2021-EGA-32), and by a bilateral mobility grant from the Hungarian Academy of Sciences (NKM2024-16/2024) and the National Science and Technology Council, Taiwan (NSTC-HAS 111-2927-I-037-501). D.L. was supported by the Cooperative Doctoral Program, 1005968.
Subjects: Q Science / természettudomány > QD Chemistry / kémia
T Technology / alkalmazott, műszaki tudományok > TX Home economics / háztartástan > TX642-TX840 Food sciences / élelmiszertudomány
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 04 Jun 2025 15:04
Last Modified: 04 Jun 2025 15:04
URI: https://real.mtak.hu/id/eprint/219763

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