Chemiluminescent enzyme-linked immunosorbent assay using monoclonal antibodies for zearalenone in food and feed

Cai, W. and Ge, M. and Xing, Y. and Sun, Y. and Wu, M. and Wang, Y. and Hu, X. (2026) Chemiluminescent enzyme-linked immunosorbent assay using monoclonal antibodies for zearalenone in food and feed. ACTA ALIMENTARIA: AN INTERNATIONAL JOURNAL OF FOOD SCIENCE, 55 (2). pp. 314-324. ISSN 0139-3006

Preview

Text 066-article-p314.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (515kB) | Preview

Abstract

A chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for the detection of zearalenone (ZEN) in maize and feed was developed by optimising antigen and antibody concentrations, as well as incubation times. The results indicated that the CL-ELISA exhibited a linear working range of 0.039–1.027 ng mL −1 , with a half-maximal inhibitory concentration (IC 50 ) of 0.187 ng mL −1 and a limit of detection (LOD) of 0.039 ng mL −1 . The method exhibited cross-reactivity ranging from 7.95 to 102.19% for five ZEN structural analogues, with no detectable cross-reactivity toward other mycotoxins or the carrier protein. The intra- and inter-assay coefficients of variation were both below 10% and recovery rates in spiked maize and feed samples ranged from 86.68 to 103.86%. In conclusion, the developed CL-ELISA is suitable for the rapid, group-specific screening of ZEN in maize and feed.

Actions (login required)

Edit Item