Cost-Effective Determination of Total Ascorbic Acid Content and Antioxidant Activity. A Food Chemistry Case Study

Csavdári, Alexandra Anna and Bogyor, Andrea and Bitay, Enikő (2024) Cost-Effective Determination of Total Ascorbic Acid Content and Antioxidant Activity. A Food Chemistry Case Study. MŰSZAKI TUDOMÁNYOS KÖZLEMÉNYEK (EN), 21. pp. 7-15. ISSN 2601-5773

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Abstract

This study investigates the cost-effective and reliable determination of total ascorbic acid (AA) content, as well as antioxidant activity, from a range of 32 alcoholic and non-alcoholic beverages. 5 cold-pressed oil sam ples and 2 types of honey were also considered. Among the iodometric methods, the back-titration of unre acted iodine with thiosulfate yielded the best results for AA content. The method proved simple, fast, reliable, and accurate, with a recovery rate of ascorbic acid exceeding 99% in 2 standardized commercial pharma ceutical products. Total antioxidant activity determination was based on the reaction of antioxidant species with ABTS, followed by a photometric assessment of the reaction’s extent. Results, expressed in terms of AA content, were good and reliable, yet in some cases less precise and accurate. Most beverages exhibited higher antioxidant activity than their respective AA content, likely influenced by other antioxidant compounds such as flavonoids and anthocyanins present in fruit-based drinks. Both methods proved useful in demonstrating the nutritional value of various easy to consume beverages, oils and honey.

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