On the electromigration of charged-fluorophore labeled oligosaccharides in polyethylene oxide solutions

Járvás, Gábor and Kerékgyártó, Márta and Guttman, András (2016) On the electromigration of charged-fluorophore labeled oligosaccharides in polyethylene oxide solutions. ELECTROPHORESIS. ISSN 0173-0835, ESSN: 1522-2683 (In Press)

Text elps5862.pdf Restricted to Registered users only Download (1MB)

Abstract

The separation mechanism of charged-fluorophore (aminopyrenetrisulfonate) labeled maltooligosaccharides with α1-4 linkage was studied in polyethylene oxide solutions (MW 300,000 Da) with special interest to possible analyte and/or network deformations as well as potential solute-matrix interactions. The electrophoretic mobilities of the APTS labeled maltooligosaccharides were found proportional with their MW-2/3 . The Arrhenius function was used to determine the activation energy needed by the labeled sugars to migrate through the separation media. With increasing solute size, the activation energy (Ea ) values decreased in polymer concentrations above the entanglement threshold of the polyethylene oxide, while showed apparently independent function at the entanglement threshold. The observed phenomenon was considered as a result of solute-matrix interaction, which could be alleviated by the addition of an organic modifier to the background electrolyte.

Actions (login required)

Edit Item